GALANIN INHIBITS RAT PANCREATIC AMYLASE RELEASE VIA CHOLINERGIC SUPPRESSION

被引：12

作者：

FLOWE, KM ^{[1
]}

LALLY, KM ^{[1
]}

MULHOLLAND, MW ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT SURG,ANN ARBOR,MI 48109

来源：

PEPTIDES | 1992年 / 13卷 / 03期

关键词：

AMYLASE; ACETYLCHOLINE; URETHANE ANESTHESIA; ACINI; LOBULES; RATS; INBRED STRAINS;

D O I：

10.1016/0196-9781(92)90079-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of galanin on pancreatic exocrine function were examined using rat pancreatic tissues. In anesthetized rats, galanin (40 mu-g/kg/h) decreased amylase secretion stimulated by 2-deoxy glucose (5.8 +/-0.1 vs. 3.1 +/- 0.1 times basal) and cholecystokinin octapeptide (21.5 +/- 0.6 vs. 16.8 +/- 0.5), while not inhibiting bethanechol-stimulated secretion. In dispersed acini, there was no effect of galanin alone (10(-8) to 10(-13) M) on amylase release, nor did galanin (10(-6) or 10(-8) M) coincubation affect amylase release stimulated by bethanechol (10(-3) to 10(-7) M) or CCK-8 (10(-8) to 10(-13) M). Using pancreatic lobules, coincubation with galanin (10(-6) M) suppressed 75 mM KCl-stimulated amylase secretion and ACh release (10.1 +/- 0.6% vs. 7.3 +/- 0.4%). Veratridine-stimulated (10(-4) M) amylase secretion and ACh release (12.4 +/- 1.7% vs. 8.5 +/-0.7%) were similarly diminished.

引用

页码：487 / 492

页数：6

共 32 条

[1] GALANIN INHIBITS AMYLASE SECRETION FROM ISOLATED RAT PANCREATIC ACINI [J].