HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR 2'-DEOXY-3'-THIACYTIDINE IN HUMAN SERUM

被引：61

作者：

HARKER, AJ ^{[1
]}

EVANS, GL ^{[1
]}

HAWLEY, AE ^{[1
]}

MORRIS, DM ^{[1
]}

机构：

[1] GLAXO INC,RES INST,DEPT CLIN PHARMACOL,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 657卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)80092-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of 2'-deoxy-3'-thiacytidine (3TC), a novel dideoxy-nucleoside analogue, in human serum is described. 3TC was extracted from serum samples using Bond Elut Certify solid-phase extraction cartridges prior to reversed-phase chromatography with UV detection. The method has been shown to be valid over the concentration range 10-5000 ng/ml using a 1-ml sample volume, both before and after heat treatment of the samples at 60 degrees C for 3 h. The method has been automated using a Zymark robot and used in the analysis of serum samples from HIV positive patients.

引用

页码：227 / 232

页数：6

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