DETERMINATION OF EQUILIBRIUM CONSTANTS OF ASSOCIATING PROTEIN SYSTEMS .3. EVALUATION OF WEIGHT FRACTION OF MONOMER FROM WEIGHT-AVERAGE PARTITION COEFFICIENT (APPLICATION TO BOVINE LIVER GLUTAMATE DEHYDROGENASE)

被引：36

作者：

CHUN, PW

KIM, SJ

STANLEY, CA

ACKERS, GK

机构：

[1] Department of Mechanical Science, University of Florida, Gainesville

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 04期

关键词：

D O I：

10.1021/bi00832a044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Procedures for evaluation of weight fraction monomer from experimental weight-average partition coefficients have been derived for several important cases of association. Since the partition coefficients are sensitive to molecular asymmetry, the molecular sieve data can be combined with molecular weight data to provide inferences regarding the mode of aggregation for an associating system. These procedures have been applied to bovine liver L-glutamate dehydrogenase. For this enzyme the correlation of molecular weight and partition coefficient data indicated a linear aggregation of subunits. The subunit association was of the “indefinite” type in which a single equilibrium constant pertains to subunit addition for all species. © 1969, American Chemical Society. All rights reserved.

引用

页码：1625 / &

共 31 条

[1]

ACKERS GK, 1967, J BIOL CHEM, V242, P3026

[2]

ACKERS GK, 1967, J BIOL CHEM, V242, P3237

[3] DETERMINATION OF STOICHIOMETRY AND EQUILIBRIUM CONSTANTS FOR REVERSIBLY ASSOCIATING SYSTEMS BY MOLECULAR SIEVE CHROMATOGRAPHY [J].