HPTLC SEPARATION OF AROMATIC ALPHA-AMINO-ACID ENANTIOMERS ON A NEW HISTIDINE-BASED STATIONARY PHASE USING LIGAND-EXCHANGE

被引：24

作者：

REMELLI, M ^{[1
]}

PIAZZA, R ^{[1
]}

PULIDORI, F ^{[1
]}

机构：

[1] UNIV FERRARA,DIPARTMENTO CHIM,VIA LUIGI BORSARI 46,I-44100 FERRARA,ITALY

来源：

CHROMATOGRAPHIA | 1991年 / 32卷 / 5-6期

关键词：

THIN-LAYER CHROMATOGRAPHY; CHIRAL SEPARATION; AMINO ACID ENANTIOMERS; LIGAND-EXCHANGE CHROMATOGRAPHY;

D O I：

10.1007/BF02276253

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new chiral ligand exchange selector for hydrophobic stationary phase modification has been synthesized by selective alkylation of L-histidine at the pyrrolic nitrogen atom in its imidazolic ring. Its performance was then tested on RP-HPTLC plates treated with copper acetate. Significant selectivity towards aromatic amino acid enantiomers, was observed. Chromatographic retention data were compared to available thermodynamic complex formation parameters for the relevant model systems in aqueous solution. Stationary and mobile phase effects on retention were studied by using different RP-HPTLC plates and various binary aqueous solvent mixtures. Optimized separation conditions for aromatic amino acid sample class are given.

引用

页码：278 / 284

页数：7

共 38 条

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