AN ENZYMATIC COLORIMETRIC METHOD FOR MEASURING NARINGIN USING 2,2'-AZINO-BIS-(3-ETHYLBENZTHIAZOLINE-6-SULFONIC ACID) (ABTS) IN THE PRESENCE OF PEROXIDASE

被引：81

作者：

ARNAO, MB ^{[1
]}

CASAS, JL ^{[1
]}

DELRIO, JA ^{[1
]}

ACOSTA, M ^{[1
]}

GARCIACANOVAS, F ^{[1
]}

机构：

[1] UNIV MURCIA,DEPT BIOQUIM & BIOL MOLEC,E-30001 MURCIA,SPAIN

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90304-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive colorimetric method for naringin estimation using 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as peroxidase substrate is described. The method is based on the coupling reaction of an ABTS radical cation with an oxidation product of naringin formed by peroxidase. This coupling reaction leads to the formation of a purple-colored compound with a maximum absorption at 560 nm. A molar absorption coefficient at this wavelength was calculated to be 13,286 ± 200 m-1 cm-1. The lowest amount of naringin that can be detected is 1 nmol. The application of this method to the quantification of naringin in grape-fruit tissues is presented. © 1990.

引用

页码：335 / 338

页数：4

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