ISOLATION OF CDNA FRAGMENTS HYBRIDIZING TO RAT BRAIN-SPECIFIC MESSENGER-RNAS

被引：14

作者：

ESPOSITO, F ^{[1
]}

AMMENDOLA, R ^{[1
]}

DUILIO, A ^{[1
]}

COSTANZO, F ^{[1
]}

GIORDANO, M ^{[1
]}

ZAMBRANO, N ^{[1
]}

DAGOSTINO, P ^{[1
]}

RUSSO, T ^{[1
]}

CIMINO, F ^{[1
]}

机构：

[1] NAPLES UNIV, FAC MED & CHIRURG 2, DIPARTIMENTO BIOCHIM & BIOTECNOL MED, VIA S PANSINI 5, I-80131 NAPLES, ITALY

来源：

DEVELOPMENTAL NEUROSCIENCE | 1990年 / 12卷 / 06期

关键词：

TISSUE SPECIFICITY; CDNA LIBRARY; BRAIN MESSENGER RNAS; SNRNP-ASSOCIATED POLYPEPTIDES;

D O I：

10.1159/000111865

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A cDNA minilibrary in pUC18 has been generated from 3-month-old rat brains. Two hundred clones were randomly selected and sequenced. Comparison with a nucleic acid and protein data bank revealed a number of cDNA fragments not homologous to any published sequence. Northern blot analyses of some of these clones yielded 5 brain-specific cDNA fragments. The full-length cDNA for one of these clones has been isolated and completely sequenced. It corresponds to an mRNA of about 1,500 nucleotides, which is present in brain and, to a lesser extent, in heart and skeletal muscle. Its expression is developmentally regulated in the rat brain from 14-day embryos to 3-month-old adults. A very recent comparison with the EMBL nucleotide sequence data bank showed that this mRNA codes for a brain-specific snRNP-associated protein.

引用

页码：373 / 381

页数：9

共 43 条

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