STRATEGIES FOR CRYSTALLIZATION OF LARGE MEMBRANE-PROTEIN COMPLEXES

被引：6

作者：

YOSHIKAWA, S

SHINZAWAITOH, K

UEDA, H

TSUKIHARA, T

FUKUMOTO, Y

KUBOTA, T

KAWAMOTO, M

FUKUYAMA, K

MATSUBARA, H

机构：

[1] UNIV TOKUSHIMA,FAC ENGN,DEPT BIOL SCI & TECHNOL,TOKUSHIMA 770,JAPAN

[2] TOTTORI UNIV,DEPT IND CHEM,TOTTORI 680,JAPAN

[3] OSAKA UNIV,FAC SCI,DEPT BIOL,TOYONAKA,OSAKA 560,JAPAN

来源：

JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期

关键词：

D O I：

10.1016/0022-0248(92)90259-L

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Crystalline cytochrome c oxidase and ubiquinol : cytochrome c oxidoreductase which diffracted X-rays at 7-8 angstrom resolution were obtained from bovine heart mitochondria. The methods for the purification and crystallization of these enzymes indicate that large membrane protein complexes are easier to purify and crystallize than smaller homologous membrane protein complexes, because the former have more hydrophilic surface than the latter. Bulky and polydispersed detergents such as Brij-35 and Tween 20 attached to the isolated complex are not always obstructive to crystallization if they are effective for stabilizing the complexes.

引用

页码：298 / 302

页数：5

共 10 条

[1]

HATEFI Y, 1985, ANNU REV BIOCHEM, V54, P1015, DOI 10.1146/annurev.bi.54.070185.005055

[2] CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDIES OF BOVINE HEART MITOCHONDRIAL CYTOCHROME-BC(1) COMPLEX [J].