DISTINCT PATTERN OF MATRIX METALLOPROTEINASE-9 AND TISSUE INHIBITOR OF METALLOPROTEINASE-1 MESSENGER-RNA EXPRESSION IN HUMAN COLORECTAL-CANCER AND LIVER METASTASES

被引：95

作者：

ZENG, ZS ^{[1
]}

GUILLEM, JG ^{[1
]}

机构：

[1] MEM SLOAN KETTERING CANC CTR,DEPT SURG,COLORECTAL SERV,NEW YORK,NY 10021

来源：

BRITISH JOURNAL OF CANCER | 1995年 / 72卷 / 03期

关键词：

IN SITU HYBRIDIZATION; MATRIX METALLOPROTEINASE; TISSUE INHIBITOR OF METALLOPROTEINASE; TYPE IV COLLAGENASE;

D O I：

10.1038/bjc.1995.376

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are perceived as essential for tumour invasion and metastasis. In the present study, we compare the topographical pattern of MMP-9 and TIMP-1 expression in colorectal cancer and liver metastases by in situ hybridisation. TIMP-1 mRNA was detected in all 26 colorectal cancers examined, while only 18 out of 26 (69.2%) were positive for MMP-9. Both MMP-9 and TIMP-1 mRNA were observed in all ten liver metastases but were absent in three adenomas and in all normal colonic mucosa and liver. There was no association between MMP-9 or TIMP-1 mRNA expression and degree of differentiation or size of rumours. MMP-9 and TIMP-1 mRNA were similarly observed in the peritumour stroma cells rather than in tumour cells themselves. MMP-9 mRNA-positive cells were round and identified as macrophages by immunostaining with an anti-macrophage antibody (KP1), while TIMP-1 mRNA was detected in spindle-shaped stromal cells. In liver metastases, MMP-9 localised within peritumour stroma or at the interface between the tumour stroma and normal liver, whereas TIMP-1 mRNA was located throughout the malignant tumour stroma. Our data demonstrate a distinct pattern of MMP-9 and TIMP-1 mRNA expression in colorectal cancer and liver metastases suggesting distinct cellular origins as well as separate patterns of regulation.

引用

页码：575 / 582

页数：8

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