THE DETERMINATION OF IGE SYNTHESIS INVITRO - METHODOLOGICAL ASPECTS

被引：3

作者：

GAMKRELIDZE, A ^{[1
]}

BJORKSTEN, B ^{[1
]}

机构：

[1] LINKOPING UNIV HOSP,FAC HLTH SCI,DEPT PEDIAT,S-58185 LINKOPING,SWEDEN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 130卷 / 01期

关键词：

Acid extraction; IgE; IgE synthesis; performed;

D O I：

10.1016/0022-1759(90)90292-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Various methods to determine net synthesis of IgE in cell cultures and to differentiate this from preformed IgE have been evaluated. The most accurate values for net synthesis were obtained by adding the IgE levels in supernatants to the level of cell-bound acid extractable IgE at the end of the culture period and subtracting the IgE levels present in cultures subject to acid treatment on day 0. © 1990.

引用

页码：9 / 13

页数：5

共 5 条

[1] IGE PRODUCTION INVITRO BY HUMAN-BLOOD MONONUCLEAR-CELLS - A COMPARISON BETWEEN ATOPIC AND NONATOPIC SUBJECTS [J].

HEMADY, Z ;

BLOMBERG, F ;

GELLIS, S ;

ROCKLIN, RE .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1983, 71 (03) :324-330

[2] HUMAN IGE SYNTHESIS INVITRO - DETECTION WITH MONOCLONAL-ANTIBODIES [J].

KNUTTIMULLER, JM ;

STADLER, BM ;

MAGNUSSON, CM ;

DEWECK, L .

ALLERGY, 1986, 41 (06) :457-467

[3] WORKSHOP ON MEASUREMENT OF INVITRO IGE SYNTHESIS AND REGULATION OF IGE SYNTHESIS [J].

MASSICOT, JG ;

ISHIZAKA, K .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1986, 77 (04) :544-554

[4]

SAMPSON HA, 1981, J IMMUNOL, V127, P829

[5]

TURNER KJ, 1983, CLIN EXP IMMUNOL, V51, P387

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