THE LENGTHS OF DNA FRAGMENTS LIGHT-INDUCED IN THE PRESENCE OF A PHOTOSENSITIZER LOCALIZED AT THE NUCLEAR-MEMBRANE OF HUMAN-CELLS

被引：21

作者：

KVAM, E

STOKKE, T

MOAN, J

机构：

[1] Department of Biophysics, Institute for Cancer Research, Oslo, Montebello

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1049卷 / 01期

关键词：

(Human cell line); DNA damage; Nuclear membrane;

D O I：

10.1016/0167-4781(90)90081-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA damage was apparently introduced selectively in the parts of DNA localized close to the nuclear membrane of human NHIK 3025 cells. This was obtained by illumination of the cells in the presence of a hydrophobic photosensitizer, Photofrin II, which was located in membrane structures but not in the nucleus. Photofrin II sensitizes DNA to light mainly via singlet oxygen, which diffuses about 0.1 μm in its intracellular lifetime. By measuring DNA unwinding in alkali after illumination or X-irradiation of cells, the distribution of the number of DNA bases between two adjacent, photodamaged DNA sites was estimated. The average length of these DNA fragments was found to be 155 kilobases (kb). © 1990.

引用

页码：33 / 37

页数：5

共 22 条

[1]

BLAZEK ER, 1984, PHOTOCHEM PHOTOBIOL, V4, P5

[2] PHOTODYNAMIC EFFECT OF HEMATOPORPHYRIN ON DNA [J].