GRACILARIA-TIKVAHIAE AGGLUTININ - PARTIAL-PURIFICATION AND PRELIMINARY CHARACTERIZATION OF ITS CARBOHYDRATE SPECIFICITY

被引：11

作者：

CHILES, TC ^{[1
]}

BIRD, KT ^{[1
]}

机构：

[1] UNIV N CAROLINA,CTR MARINE SCI RES,WILMINGTON,NC 28403

来源：

CARBOHYDRATE RESEARCH | 1990年 / 207卷 / 02期

关键词：

D O I：

10.1016/0008-6215(90)84059-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A potent agglutinin of rabbit and sheep red blood cells, obtained from the red alga Gracilaria tikvahiae, was purified by ammonium sulfate fractionation, ion exchange, gel filtration, and hydroxylapatite chromatography. Human A and B blood group erythrocytes were also agglutinated, whereas human O blood group erythrocytes were not agglutinated. The hemagglutination titer was not significantly affected by the addition of EDTA or the divalent cations Ca2+, Mg2+, or Mn2+. The carbohydrate specificity was characterized by hemagglutination inhibition using various monosaccharides, glycoproteins, and glycopeptides. The results suggested that the agglutinin has affinity for N-acetylneuraminic acid as well as glycoconjugates containing N-acetylneuraminic acid. © 1990.

引用

页码：319 / 326

页数：8

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