SIZE AND CHARGE PROPERTIES OF THE PECTIC AND CELLULOLYTIC ENZYMES IN A COMMERCIAL ENZYME PREPARATION

The size and charge properties of the pectic and cellulolytic enzymes in a commercial preparation were studied using with isoelectric focusing, nondenaturating polyacrylamide gradient gel electrophoresis, titration curve analysis and a two-dimensional technique. Enzyme activity was detected with the zymogram technique. Five major isoenzymes of endo-poly-galacturonase were detected having isoelectric points (pI) of 3.2, 3.6, 3.7, 4.4, and 4.5. These separate into two major size species after PAGGE (M(r) 28000 and 48000). -Endo-pectinlyase was detected at pI 3.6/M(r) 38000 after two-dimensional electrophoresis. - Pectin esterase was found to have a pI of 3.5 and a M(r) of 27000. -Endo-Cx-cellulases were found at pI 3.1-3.2/M(r) 38000; pI 3.5/M(r) 27000; pI 3.6-3.8/M(r) 80000; pI 5.6/M(r) 70000.