DNA DAMAGE PRODUCED IN V79 CELLS BY DNA-INCORPORATED I-123 - A COMPARISON WITH I-125

被引：45

作者：

MAKRIGIORGOS, GM ^{[1
]}

BERMAN, RM ^{[1
]}

BARANOWSKAKORTYLEWICZ, J ^{[1
]}

BUMP, E ^{[1
]}

HUMM, JL ^{[1
]}

ADELSTEIN, SJ ^{[1
]}

KASSIS, AI ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT RADIAT THERAPY,BOSTON,MA 02115

来源：

RADIATION RESEARCH | 1992年 / 129卷 / 03期

关键词：

D O I：

10.2307/3578031

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The neutral elution technique (pH 9.6) has been used to compare the damage produced in the DNA of V79 cells following the decay of the Auger-electron emitters 123I and 125I incorporated into DNA in the form of 5- [123I/125I]iodo-2'-deoxyuridine (123IdU, 125IdU) or after 60Co γ irradiation. Elution profiles of retained radioactivity versus eluted volume were generally found to be nonlinear and partially dependent on the cell treatment prior to elution. Plots of a suitable function of radioactivity retention on the filters versus total decays for 123I or 125I and dose (Gy) for γ radiation were linear. Assuming that each 125I decay produces one double-strand break (DSB), the 60Co efficiency in DSB production was found to be 53.4 DSBs/Gy/cell (or 17.8 DSBs/Gy/1012 Da), in agreement with values reported previously. The decay of 123I led to 0.74 DSB/decay/cell if decays occurring only during nonrepair conditions were counted. This DSB production efficiency is in agreement with Monte Carlo semiempirical models of the action of radiation on DNA.

引用

页码：309 / 314

页数：6

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