CROSS-REACTIVE AND SEROTYPE-SPECIFIC ANTIBODIES AGAINST FOOT-AND-MOUTH-DISEASE VIRUS GENERATED BY DIFFERENT REGIONS OF THE SAME SYNTHETIC PEPTIDE

被引:10
作者
DOEL, TR [1 ]
DOEL, CMFA [1 ]
STAPLE, RF [1 ]
DIMARCHI, R [1 ]
机构
[1] ELI LILLY & CO, LILLY RES LAB, INDIANAPOLIS, IN 46285 USA
关键词
D O I
10.1128/JVI.66.4.2187-2194.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Synthetic peptides based on the VP1 proteins of two serotypes of foot-and-mouth disease virus (FMDV) and having the general formula C-C-(200-213)-P-P-S-(141-158)-P-C-G induce heterologous as well as homologous protection against challenge. Substitution of the sequence consisting of residues 200 to 213 (200-213 sequence) with a second copy of the homologous 141-158 sequence (i.e., homodimers) resulted in failure of either serotype peptide to protect heterologously. The antiviral and antipeptide titers of sera from guinea pigs immunized with the homodimeric 141-158 peptides showed serotype specificity and, with the data from the heterodimeric peptide vaccines, suggested that the C-terminal 141-158 sequence was more effectively recognized by the immune system than the N-terminal sequence. Whereas heterologous antiviral titers as measured by enzyme-linked immunosorbent assay and virus neutralization tests have not been observed with sera from cross-protected animals, epitope-mapping studies established that there was heterologous recognition of an octapeptide within the 200-213 sequence. That the 200-213 sequence was required for the induction of heterologous protection was also confirmed with a number of peptides, including hybrids based on the 200-213 sequence of one virus and the 141-158 sequence of a second virus. Thus, peptides of the general formula given above induce serotype-specific and serotype-cross-reactive protective antibodies and are unique in their induction of significant levels of heterologous protection, a property which has never been reported for whole FMDV vaccines.
引用
收藏
页码:2187 / 2194
页数:8
相关论文
共 20 条
[1]   THE 3-DIMENSIONAL STRUCTURE OF FOOT-AND-MOUTH-DISEASE VIRUS AT 2.9-A RESOLUTION [J].
ACHARYA, R ;
FRY, E ;
STUART, D ;
FOX, G ;
ROWLANDS, D ;
BROWN, F .
NATURE, 1989, 337 (6209) :709-716
[2]   NEUTRALIZING EPITOPES OF TYPE-O FOOT-AND-MOUTH-DISEASE VIRUS .1. IDENTIFICATION AND CHARACTERIZATION OF 3 FUNCTIONALLY INDEPENDENT, CONFORMATIONAL SITES [J].
BARNETT, PV ;
OULDRIDGE, EJ ;
ROWLANDS, DJ ;
BROWN, F ;
PARRY, NR .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :1483-1491
[3]   PROTECTION AGAINST FOOT-AND-MOUTH-DISEASE BY IMMUNIZATION WITH A CHEMICALLY SYNTHESIZED PEPTIDE PREDICTED FROM THE VIRAL NUCLEOTIDE-SEQUENCE [J].
BITTLE, JL ;
HOUGHTEN, RA ;
ALEXANDER, H ;
SHINNICK, TM ;
SUTCLIFFE, JG ;
LERNER, RA ;
ROWLANDS, DJ ;
BROWN, F .
NATURE, 1982, 298 (5869) :30-33
[4]  
COLLEN T, 1991, J IMMUNOL, V146, P749
[5]   ORIENTATION OF EPITOPES INFLUENCES THE IMMUNOGENICITY OF SYNTHETIC PEPTIDE DIMERS [J].
COX, JH ;
IVANYI, J ;
YOUNG, DB ;
LAMB, JR ;
SYRED, AD ;
FRANCIS, MJ .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (12) :2015-2019
[6]   PROTECTION OF CATTLE AGAINST FOOT-AND-MOUTH-DISEASE BY A SYNTHETIC PEPTIDE [J].
DIMARCHI, R ;
BROOKE, G ;
GALE, C ;
CRACKNELL, V ;
DOEL, T ;
MOWAT, N .
SCIENCE, 1986, 232 (4750) :639-641
[7]  
DOEL TJ, UNPUB
[8]   IMMUNIZATION AGAINST FOOT-AND-MOUTH-DISEASE WITH SYNTHETIC PEPTIDES REPRESENTING THE C-TERMINAL REGION OF VP1 [J].
DOEL, TR ;
GALE, C ;
BROOKE, G ;
DIMARCHI, R .
JOURNAL OF GENERAL VIROLOGY, 1988, 69 :2403-2406
[9]   HETEROTYPIC PROTECTION INDUCED BY SYNTHETIC PEPTIDES CORRESPONDING TO 3 SEROTYPES OF FOOT-AND-MOUTH-DISEASE VIRUS [J].
DOEL, TR ;
GALE, C ;
AMARAL, CMCFD ;
MULCAHY, G ;
DIMARCHI, R .
JOURNAL OF VIROLOGY, 1990, 64 (05) :2260-2264
[10]  
FLYNN JN, IN PRESS VET IMMUNOL