MOLECULAR MASS DETERMINATION AND ASSAY OF VENOM HYALURONIDASES BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS

被引：66

作者：

CEVALLOS, MA

NAVARRODUQUE, C

VARELAJULIA, M

ALAGON, AC

机构：

[1] UNIV NACL AUTONOMA MEXICO, INST BIOTECNOL, AP 510-3, CUERNAVACA 62271, MORELOS, MEXICO

[2] LA NAUYACA SERPENTARIUM, MEXICO CITY 09440, DF, MEXICO

来源：

TOXICON | 1992年 / 30卷 / 08期

关键词：

D O I：

10.1016/0041-0101(92)90392-I

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We describe a procedure for molecular mass determination of hyaluronidases present in animal venoms from different families. Hyaluronidases can be revealed, following their electrophoretic separation in sodium dodecyl sulfate-polyacrylamide gel containing hyaluronic acid, by incubating the gel in Triton X-100 to remove sodium dodecyl sulfate and restore in situ enzyme activity. This method allows the detection of as little as 0.025 turbidity-reducing units after 2 hr incubation. All the hyaluronidases from the analyzed invertebrate venoms had a mass below 50,000 and showed only one component, while those from vertebrate venoms were more than 60,000 and in many instances contained more than one form.

引用

页码：925 / 930

页数：6

共 18 条

[1]

ALAGON AC, 1983, BIOTECNOLOGIA ENZIMA, P125

[2] CHONDROMUCOPROTEIN-DEGRADING ENZYMES [J].