PRELIMINARY CRYSTALLOGRAPHIC STUDY OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM TRYPANOSOMA-BRUCEI

被引：5

作者：

PHILLIPS, C ^{[1
]}

BARRETT, MP ^{[1
]}

GOVER, S ^{[1
]}

LEPAGE, RWF ^{[1
]}

ADAMS, MJ ^{[1
]}

机构：

[1] UNIV CAMBRIDGE, DEPT PATHOL, CAMBRIDGE CB1 2QP, ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 233卷 / 02期

基金：

英国惠康基金;

关键词：

6-PHOSPHOGLUCONATE DEHYDROGENASE; TRYPANOSOMA-BRUCEI; CRYSTALLIZATION; X-RAY DIFFRACTION; ROTATION FUNCTION;

D O I：

10.1006/jmbi.1993.1510

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have obtained well-ordered single crystals of 6-phosphogluconate dehydrogenase, an enzyme of the oxidative branch of the pentose phosphate pathway, from Trypanosoma brucei. The crystals are trigonal rhombs with unit cell dimensions a = b = 135.1 Å, c = 116.7 Å and belong to one of the enantiomorphic pair of space groups P3121/P3221. X-ray diffraction to better than 2.8 Å has been recorded using a rotating anode CuKα source. Elucidation of the three-dimensional structure of the T. brucei enzyme will, by indicating structural differences from the known sheep enzyme structure, aid the design of mutants to probe the active site. Knowledge of the structure will also assist in assessing the potential use of rationally designed compounds to inhibit this enzyme specifically. © 1993 Academic Press Limited.

引用

页码：317 / 321

页数：5

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