NEW HIGHLY-SENSITIVE CHROMOGENIC ALKALINE-PHOSPHATASE REAGENT - 5,10,15,20-TETRAKIS(4-PHOSPHONOOXYPHENYL)PORPHINE

被引：4

作者：

KAWAKAMI, T

IGARASHI, S

机构：

[1] Department of Materials Science, Faculty of Engineering, Ibaraki University, Nakanarusawa, Hitachi

来源：

ANALYTICAL LETTERS | 1994年 / 27卷 / 11期

关键词：

WATER-SOLUBLE PORPHYRIN; ALKALINE PHOSPHATASE; ENZYME REACTION; SPECTROPHOTOMETRIC DETERMINATION; 5,10,15,20-TETRAKIS(4-PHOSPHONOOXYPHENYL)PORPHINE;

D O I：

10.1080/00032719408007236

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new water-soluble porphyrin having the phosphate group, 5,10,15,20-tetrakis(4-phosphonooxyphenyl)porphine, was used as an ultra highly-sensitive chromogenic reagent of alkaline phosphatase enzyme activity. In the spectrophotometric determination of trace amounts of alkaline phosphatase with this reagent, the calibration curve was linear in the concentration range of 3.2 x 10(-5) to 6.4 x 10(-4) g/l and detection limit {S/N=3} of alkaline phosphatase was 8.0 X 10(-8) g/l (10(-12)M) for 30 min and the relative standard deviation was 3.5% (2.0 X 10(-5) g/l ALP, 10 determinations). This reagent was also stable for seven days in aqueous solution (37 degrees C, pH=9.4).

引用

页码：2083 / 2089

页数：7