MICROSPECTROPHOTOMETRIC CHARACTERIZATION OF AROMATIC CONSTITUENTS IN CELL-WALLS OF HARD AND SOFT WHEATS

被引:18
作者
AKIN, DE
机构
[1] United States Department of Agriculture-Agricultural Research Service, Richard B Russell Agricultural Research Center, Athens, Georgia, 30604
关键词
WHEAT; LIGNIN; FERULIC ACID; HISTOCHEMISTRY; TRITICUM AESTIVUM; UV ABSORPTION; CELL WALLS;
D O I
10.1002/jsfa.2740680212
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Cell walls of epidermis, hypodermis, nucellar epidermis, aleurone, and endosperm in hard and soft wheat (Triticum aestivum L) kernels were evaluated for aromatic constituents using ultraviolet (UV) absorption microspectrophotometry. Wheat varieties sampled were soft red winter Caldwell, hard red winter varieties Tam 200 and Karl, and hard red spring varieties Len and Yecora rojo. Spectra of cell walls of epidermis and hypodermis (beeswing cells) suggested larger amounts and a greater degree of polymerization of aromatic constituents in these walls compared to those of other cell types. Results also suggested the presence of ester-linked ferulic acid. Nucellar epidermal walls also had polymerized aromatics and ferulic acid, but amounts appeared to be much less than in the walls of the beeswing cells. UV spectra of aleurone walls suggested that the predominant aromatic was ester-linked ferulic acid; anticlinal walls had substantially more aromatic constituents than outer or inner periclinal walls. Endosperm walls lacked any indication of aromatics by this method. Grain hardness did not appear to be related to cell wall aromatics. UV absorption microspectrophotometry indicated significant variations in wall aromatics of different cell types of wheat grains.
引用
收藏
页码:207 / 214
页数:8
相关论文
共 30 条
[1]   OZONE TREATMENT OF FORAGE - STRUCTURE AND DIGESTIBILITY OF DIFFERENT TYPES OF LIGNIFIED CELL-WALLS [J].
AKIN, DE ;
MORRISON, WH .
CROP SCIENCE, 1988, 28 (02) :337-342
[2]  
AKIN DE, 1992, FOOD STRUCT, V11, P259
[3]   DIAZONIUM COMPOUNDS LOCALIZE GRASS CELL-WALL PHENOLICS - RELATION TO WALL DIGESTIBILITY [J].
AKIN, DE ;
HARTLEY, RD ;
MORRISON, WH ;
HIMMELSBACH, DS .
CROP SCIENCE, 1990, 30 (05) :985-989
[4]   MICROSPECTROPHOTOMETRY AND DIGESTIBILITY OF ALKALI-TREATED WALLS IN BERMUDAGRASS CELL-TYPES [J].
AKIN, DE ;
HARTLEY, RD .
CROP SCIENCE, 1992, 32 (05) :1116-1122
[5]   UV ABSORPTION MICROSPECTROPHOTOMETRY AND DIGESTIBILITY OF CELL-TYPES OF BERMUDAGRASS INTERNODES AT DIFFERENT STAGES OF MATURITY [J].
AKIN, DE ;
HARTLEY, RD .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1992, 59 (04) :437-447
[6]   LECTINS AS CYTOCHEMICAL PROBES OF THE DEVELOPING WHEAT-GRAIN .2. REACTION OF WHEAT-GERM LECTIN WITH THE NUCELLAR EPIDERMIS [J].
BALDO, BA ;
BONIFACE, PA ;
SIMMONDS, DH .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1982, 9 (06) :663-675
[7]   ASSAY FOR TRANS-PARA-COUMAROYL ESTERASE USING A SPECIFIC SUBSTRATE FROM PLANT-CELL WALLS [J].
BORNEMAN, WS ;
HARTLEY, RD ;
HIMMELSBACH, DS ;
LJUNGDAHL, LG .
ANALYTICAL BIOCHEMISTRY, 1990, 190 (01) :129-133
[8]   LIGNIN CONSTITUENTS AND CELL-WALL DIGESTIBILITY OF GRASS AND LEGUME STEMS [J].
BUXTON, DR ;
RUSSELL, JR .
CROP SCIENCE, 1988, 28 (03) :553-558
[9]   ENZYMATIC DEGRADATION AND NATURE OF THE ENDOSPERM CELL-WALLS OF GERMINATING SORGHUMS AND BARLEY [J].
ETOKAKPAN, OU .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1993, 61 (04) :389-393
[10]  
FULCHER R G, 1982, Food Microstructure, V1, P167