APPEARANCE OF ARGININE AND ARGINASE IN PUMPKIN COTYLEDONS - CHARACTERIZATION OF ARGINASE

被引：42

作者：

SPLITTST.WE

机构：

[1] Department of Horticulture, University of Illinois, Urbana

来源：

PHYTOCHEMISTRY | 1969年 / 8卷 / 04期

关键词：

D O I：

10.1016/S0031-9422(00)85847-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During germination an increase in free arginine and arginase activity occurs in the cotyledons of pumpkin seedlings grown either in the light or dark, with the increase in enzyme activity paralleling the increase in free arginine content. Arginase was purified 9-fold from etiolated cotyledons and has a pH optimum of 9·5 in pyrophosphate buffer. The velocity of the reaction was linear up to 2 hr with a requirement for manganese. Iron, cobalt and copper gave a slight stimulation while other metals gave no stimulation. Arginase has a Km of 26 μM with arginine as the substrate. © 1970.

引用

页码：753 / &

共 17 条

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