GLYCOGEN-SYNTHESIS IN FUNGUS NEUROSPORA-CRASSA

被引：10

作者：

FONTANA, JD

KRISMAN, CR

机构：

[1] INST INVEST BIOQUIM FUNDAC CAMPOMAR,BUENOS AIRES 1428,ARGENTINA

[2] FAC CIENCIAS EXACTAS & NAT,BUENOS AIRES 1428,ARGENTINA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 540卷 / 02期

关键词：

D O I：

10.1016/0304-4165(78)90130-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-[14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a 'primer' of the α 1 → 4 glucan type. © 1978.

引用

页码：183 / 189

页数：7

共 19 条

[1] INITIATION OF GLYCOGEN BIOSYNTHESIS IN ESCHERICHIA-COLI [J].