REFOLDING BEHAVIOR OF A KINETIC INTERMEDIATE OBSERVED IN THE LOW PH UNFOLDING OF RIBONUCLEASE-A

被引：31

作者：

HAGERMAN, PJ ^{[1
]}

SCHMID, FX ^{[1
]}

BALDWIN, RL ^{[1
]}

机构：

[1] STANFORD UNIV, MED CTR, DEPT BIOCHEM, STANFORD, CA 94305 USA

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 02期

关键词：

D O I：

10.1021/bi00569a009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A transient intermediate (I3) observed previously in the unfolding of RNase A was studied by employing a sequential mixing instrument to selectively populate this species. The refolding behavior of this species was determined and the kinetics of its formation further characterized. Formation of I3 represents the earliest detectable change in unfolding. The loss of the 2''CMP binding site occurs in parallel with the exposure of the interior of the protein to solvent. I3 is distinct from previously described intermediates in refolding. Overall condensation of the protein to exclude solvent from the interior, and the formation of a substrate binding site, takes place in approximately 30 ms (pH 5.8, 47.degree. C), indicating that the formation of native structure can take place faster than had previously been supposed.

引用

页码：293 / 297

页数：5

共 14 条

[1]

Ballou D. P., 1971, THESIS U MICHIGAN

[2]

BEAVEN GH, 1955, NUCLEIC ACIDS, V1, P513

[3] NUCLEAR MAGNETIC-RESONANCE EVIDENCE FOR A STRUCTURAL INTERMEDIATE AT AN EARLY STAGE IN REFOLDING OF RIBONUCLEASE-A [J].

BLUM, AD ;

SMALLCOMBE, SH ;

BALDWIN, RL .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 118 (03) :305-316

[4] BOTH FAST AND SLOW REFOLDING REACTIONS OF RIBONUCLEASE-A YIELD NATIVE ENZYME [J].

GAREL, JR ;

BALDWIN, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (12) :3347-3351

[5] HEAT-UNFOLDED STATE OF RIBONUCLEASE-A IS AN EQUILIBRIUM MIXTURE OF FAST AND SLOW REFOLDING SPECIES [J].

GAREL, JR ;

BALDWIN, RL .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 94 (04) :611-&

[6] PHYSICAL DIFFERENCE BETWEEN FAST-REFOLDING AND SLOW-REFOLDING FORMS OF NITROTYROSYL RIBONUCLEASE-A - PK VALUES OF NITROTYROSYL GROUPS [J].

GAREL, JR ;

BALDWIN, RL .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 94 (04) :621-&

[7] QUANTITATIVE TREATMENT OF KINETICS OF FOLDING TRANSITION OF RIBONUCLEASE-A [J].

HAGERMAN, PJ ;

BALDWIN, RL .

BIOCHEMISTRY, 1976, 15 (07) :1462-1473

[8]

HAGERMAN PJ, 1977, THESIS STANFORD U

[9]

Hammes G G, 1968, Adv Protein Chem, V23, P1, DOI 10.1016/S0065-3233(08)60399-X

[10] TEST OF EXTENDED 2-STATE MODEL FOR KINETIC INTERMEDIATES OBSERVED IN FOLDING TRANSITION OF RIBONUCLEASE-A [J].

NALL, BT ;

GAREL, JR ;

BALDWIN, RL .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 118 (03) :317-330

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