EFFECT OF OUABAIN ON SINDBIS VIRUS-REPLICATION IN OUABAIN-SENSITIVE AND OUABAIN-RESISTANT AEDES-ALBOPICTUS CELLS (SINGH)

The effects of ouabain on Sindbis virus replication in cloned sublines of ouabain-sensitive (B1C4) and -resistant (Oua5) Aedes albopictus cells have been studied. The addition of ouabain (0.1 mM) to B1C4 cells 1 hr after infection reduced the yield of infectious virus 500-fold. The same concentration had no effect on the yield from Oua.5 cells. Analysis of [3H]-uridine-labeled RNA by gel electrophoresis showed that ouabain markedly inhibited Sindbis virus-specific RNA synthesis in B1C4 cells but only partially inhibited host RNA synthesis. In Oua5 cells, ouabain had little effect on either host or viral RNA synthesis. A close correlation was observed between the inhibition of host protein synthesis by ouabain and the inhibition of virus replication. © 1978.