EPITOPE MAPPING OF THE MYCOBACTERIUM-BOVIS SECRETORY PROTEIN-MPB70 USING OVERLAPPING PEPTIDE ANALYSIS

被引：46

作者：

RADFORD, AJ ^{[1
]}

WOOD, PR ^{[1
]}

BILLMANJACOBE, H ^{[1
]}

GEYSEN, HM ^{[1
]}

MASON, TJ ^{[1
]}

TRIBBICK, G ^{[1
]}

机构：

[1] COMMONWEALTH SERUM LABS,PARKVILLE,VIC 3052,AUSTRALIA

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

关键词：

D O I：

10.1099/00221287-136-2-265

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene encoding the major Mycobacterium bovis secreted protein MPB70 was cloned and isolated from a DNA library in λ EMBL 3, and the restriction map of the area of chromosome containing the gene ascertained. After sub-cloning, the complete DNA sequence and predicted amino acid sequence were determined, and from this information a series of overlapping octapeptides encoding all possible linear epitopes of eight or less amino acids were synthesized. These peptides were probed with monoclonal antibodies specific for M. bovis and with sera from M. bovis-infected cattle. Epitopes defined by this technique were then examined using a substitution analysis that allowed the significance of each amino acid in the putative epitope to be quantified, and the exact specificity of the antibody response for the epitope determined.

引用

页码：265 / 272

页数：8

共 37 条

[1] CHARACTERIZATION OF THE SECRETED ANTIGENS OF MYCOBACTERIUM-BOVIS BCG - COMPARISON OF THE 46-KILODALTON DIMERIC PROTEIN WITH PROTEINS MPB64 AND MPB70 [J].