AFFINITY CHROMATOGRAPHY OF BOVINE PANCREATIC RIBONUCLEASE-A

被引：108

作者：

WILCHEK, M

GORECKI, M

机构：

[1] Department of Biophysics, Weizmann Institute of Science, Rehovot

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 11卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00799.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5′‐(4‐Aminophenyl‐phosphoryl)‐uridine‐2′(3′)‐phosphate was synthesized and coupled to Sepharose by activation with cyanogen bromide. This conjugate was used as a specific adsorbent for purification of ribonuclease A by affinity chromatography. Totally reduced and oxidized RNase preparations which are enzymatically inactive were not adsorbed or retarded by the RNase‐specific column, while S‐protein is strongly retarded. Copyright © 1969, Wiley Blackwell. All rights reserved

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页码：491 / &

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