GENERATION AND APPLICATION OF A MONOCLONAL-ANTIBODY RAISED AGAINST A RECOMBINANT CYTOMEGALOVIRUS-SPECIFIC POLYPEPTIDE

被引：23

作者：

JAHN, G

HARTHUS, HP

BROKER, M

BORISCH, B

PLATZER, B

PLACHTER, B

机构：

[1] BEHRINGWERKE AG, W-3550 MARBURG, GERMANY

[2] UNIV BERN, INST PATHOL, CH-3000 BERN, SWITZERLAND

来源：

KLINISCHE WOCHENSCHRIFT | 1990年 / 68卷 / 20期

关键词：

Cytomegalovirus; Immunofluorescence; Immunohistochemistry; Monoclonal antibody; Phosphorylated protein pp150; Procaryotic expression; Recombinant antigen;

D O I：

10.1007/BF01646545

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Procedures for diagnostics of cytomegalovirus infections include histopathology, cell culture, serology, and direct detection of viral antigens or nucleic acids within infected cells or tissues. In order to develop a new diagnostic reagent for viral antigen detection, we generated a mouse monoclonal antibody. This antibody was raised against a recombinant antigen representing part of the large phosphorylated structural protein pp150 of human cytomegalovirus. The monoclonal antibody was shown to be useful for antigen detection by immunofluorescence and immunoenzymatic staining in infected cells from cell culture as well as from infected organs. The antibody proved to be reactive even in paraffin-embedded sections from tissue specimens. © 1990 Springer-Verlag.

引用

页码：1003 / 1007

页数：5

共 21 条

[1]

BORISCH B, 1988, VIRCHOWS ARCH B, V55, P93

[2] DEFINED MONOCLONAL-ANTIBODIES TO ESCHERICHIA-COLI BETA-GALACTOSIDASE AS A TOOL FOR CHARACTERIZATION OF RECOMBINANT EXPRESSION PRODUCTS [J].