PROTON NMR SPECTROSCOPIC STUDIES ON THE METABOLISM AND BIOCHEMICAL EFFECTS OF HYDRAZINE INVIVO

被引：41

作者：

SANINS, SM

TIMBRELL, JA

ELCOMBE, C

NICHOLSON, JK

机构：

[1] UNIV LONDON, BIRKBECK COLL,DEPT CHEM,ANALYT SCI GRP, GORDON HOUSE,29 GORDON SQ, LONDON WC1H OPP, ENGLAND

[2] UNIV LONDON, SCH PHARM, TOXICOL UNIT, LONDON WC1N 1AX, ENGLAND

[3] ICI PLC, CENT TOXICOL LAB, MACCLESFIELD SK10 4TG, CHESHIRE, ENGLAND

来源：

ARCHIVES OF TOXICOLOGY | 1992年 / 66卷 / 07期

基金：

英国惠康基金;

关键词：

HYDRAZINE; PROTON NMR; URINALYSIS; MECHANISMS OF TOXICITY;

D O I：

10.1007/BF01970674

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The metabolism and disposition of hydrazine and its effects on endogenous metabolites has been studied in rats by the use of high resolution proton NMR spectroscopy of urine. Several metabolites of hydrazine were detected, notably acetyl- and diacetylhydrazine and a cyclised metabolite which results from a hydrazone formed from 2-oxoglutarate and hydrazine. Effects of hydrazine on endogenous metabolites in urine and plasma were also observed; notably a dose-related increase in urinary taurine, a dose-related increase in urinary and plasma lactate, increases in urinary alpha-alanine, beta-alanine, methylamine and a decrease in urinary 2-oxoglutarate. This study has indicated the utility of using high resolution proton NMR spectroscopy to analyse urine for both metabolites and endogenous compounds after exposure of animals to toxic substances.

引用

页码：489 / 495

页数：7

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