DELETIONS WITHIN THE DNA RECOGNITION SUBUNIT OF M.ECOR124I THAT IDENTIFY A REGION INVOLVED IN PROTEIN-PROTEIN INTERACTIONS BETWEEN HSDS AND HSDM

被引:19
作者
ABADJIEVA, A [1 ]
WEBB, M [1 ]
PATEL, J [1 ]
ZINKEVICH, V [1 ]
FIRMAN, K [1 ]
机构
[1] UNIV PORTSMOUTH, SCH BIOL SCI, BIOPHYS LAB, PORTSMOUTH PO1 2DT, HANTS, ENGLAND
基金
英国惠康基金;
关键词
SUBUNIT ASSEMBLY; PROTEIN-PROTEIN INTERACTIONS; RESTRICTION AND MODIFICATION; ENDONUCLEASES AND DELETION MAPPING;
D O I
10.1006/jmbi.1994.1471
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The DNA recognition subunit (HsdS) of type I restriction endonucleases can be divided into domains by means of amino acid identity between subunits from the same family. It has been proposed that DNA-protein interactions occur within the variable domains of the subunit and that protein-protein interactions involve the conserved domains. We have constructed a number of deletion mutants of HsdS that have allowed us to investigate protein-protein interactions. Using a combination of a ''competitive'' complementation assay and the ability of HsdM to ''solubilize'' HsdS, we have defined a region within the central conserved domain of HsdS that is responsible for HsdS-HsdM interaction. Computer analysis of amino acid identity between the N-terminal half and the C-terminal half of HsdS identifies a region (repeated in both conserved domains), one copy of which overlaps the region we have identified as essential for HsdS-HsdM interactions, which may be responsible for such protein-protein interactions.
引用
收藏
页码:35 / 43
页数:9
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