A QUANTITATIVE MICRO METHOD FOR ESTIMATION OF AMINE OXIDES

被引：15

作者：

MITCHELL, CH

ZIEGLER, DM

机构：

[1] Clayton Foundation Biochemical Institute, The University of Texas at Austin, Austin

[2] Department of Chemistry, The University of Texas at Austin, Austin

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90177-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method of estimating small amounts of the N-oxides of N,N-dimethylalkylamines and saturated N-methylheterocyclicamines is described. These amine oxides are known to react with SO2 in cold aqueous solution to produce an SO2 addition product that is immediately hydrolyzed to yield an aldehyde and a secondary amine. The aldehyde concentration, which can be estimated by a standard colorimetric method, is a function of the original amine oxide concentration. Amine oxides produced during enzymic N-oxidation of the tertiary amines can be quantitatively estimated by this method. The conditions required to extract quantitatively the amine oxide from the enzymic reaction mixture are described. © 1969.

引用

页码：261 / &

共 14 条

[1]

BAKER JR, 1963, BIOCHIM BIOPHYS ACTA, V71, P58

[2] The behavior of trimethylamino, trimethylammino-sulfur trioxide and trimethylamine oxide toward sulfur dioxide [J].