PHOSPHOTYROSINE-CONTAINING PROTEINS IN DICTYOSTELIUM-DISCOIDEUM

被引：17

作者：

SCHWEIGER, A

MIHALACHE, O

MUHR, A

ADRIAN, I

机构：

[1] Max-Planck-Institut für Biochemie, 8033 Martinsried bei München, Am Klopferspitz

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Dictyostelium discoideum; Phosphotyrosine antibody; Protein-tyrosine kinase;

D O I：

10.1016/0014-5793(90)81007-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and inununoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and, in addition, a 82 kDa one became phosphorylated on tyrosine when the immunoprecipitate was incubated with [γ-32p]ATP. In preparations from differentiating cells the intensity of the label was increased in the 60 kDa band and decreased in the 107 and 205-220 kDa bands. © 1990.

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页码：199 / 202

页数：4

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