VITAMIN-E RADICAL REACTION WITH ANTIOXIDANTS IN RAT-LIVER MEMBRANES

被引:17
作者
HIRAMATSU, M [1 ]
VELASCO, RD [1 ]
PACKER, L [1 ]
机构
[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,MEMBRANE BIOENERGET GRP,251 LSA,BERKELEY,CA 94720
关键词
VITAMIN-E; TOCOPHEROXY RADICAL; MEMBRANES; TJ-960; ASCORBATE; GLUTATHIONE; FREE RADICALS;
D O I
10.1016/0891-5849(90)90122-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Japanese herbal medicine Sho-saiko-to-go-keishi-ka-shakuyaku-to (TJ-960) has been demonstrated to have an antioxidant action by quenching free radicals. The effects of TJ-960 on the tocopheroxy radicals generated by an arachidonic acid and lipoxygenase oxidation system were compared with those of the ascorbate and glutathione in vitamin E-enriched rat liver microsomes and submitochondrial membrane particles (SMP). Using electron spin resonance spectrometry, the disappearance of the tocopheroxy radicals after addition of glutathione and ascorbate was detected in microsomes and SMP, with ascorbate displaying a more potent action than glutathione. Addition of TJ-960 demonstrated a similar effect on the tocopheroxy radicals in microsomes and SMP. In the presence of TJ-960, ascorbate, and glutathione, the loss of vitamin E in the vitamin E-enriched microsomes of rat liver undergoing oxidation was slowed down. In this paper, we introduced TJ-960 as another replenisher of vitamin E in membrane, increasing the membrane's resistance against oxidative damage.
引用
收藏
页码:459 / 464
页数:6
相关论文
共 21 条
[1]  
Burton, Ingold, Vitamin E: application of the principles of physical organic chemistry to the exploration of its structure and function, Acc. Chem. Res., 19, pp. 194-201, (1986)
[2]  
Packer, Maguire, Melhorn, Serbinova, Kagan, Mitochondria and microsomal membranes have a free radical reductase activity that prevent chromanoxyl radical accumulation, Biochem. Biophys. Res. Commun., 159, pp. 229-235, (1989)
[3]  
Mehlhorn, Sumida, Packer, Tocopheroxyl radical persistence and tocopherol consumption in liposomes and in vitamin E-enriched rat liver mitochondria and microsomes, J. Biol. Chem., 264, pp. 13448-13460, (1989)
[4]  
Niki, Interaction of ascorbate and alpha-tocopherol, Ann. N.Y. Acad. Sci., 498, pp. 186-199, (1987)
[5]  
McCay, Vitamin E: interactions with free radicals and ascorbate, Ann. Rev. Nutr., 5, pp. 323-340, (1985)
[6]  
Scarpa, Rigo, Maiorino, Urshini, Gregolin, Formation of alpha-tocopherol by ascorbate during peroxidation of phosphatidylcholine liposomes, Biochim. Biophys. Acta, 801, pp. 215-219, (1984)
[7]  
Hiramatsu, Edamatsu, Kohno, Mori, Scavenging of free radicals by Sho-saiko-to-go-keishi-ka-shakuyaku-to (TJ-960), Recent advances in the pharmacology of Kampo (Japanese herbal) medicines, (1988)
[8]  
Bielski, Richter, Chan, Some properties of the ascorbate free radicals, Annals of the New York Academy of Sciences, 258, pp. 231-237, (1985)
[9]  
Nishikimi, Oxidation of ascorbic acid with superoxide anion generated by the xanthine-xanthine oxidase system, Biochem. Biophys. Res. Commun., 63, pp. 463-468, (1975)
[10]  
Hemila, Roberts, Wikstrom, Activated polymorphonuclear leucocytes consume vitamin E, FEBS Lett., 178, pp. 25-30, (1985)