CDNA SEQUENCE OF A DROSOPHILA-MELANOGASTER GENE, DFUR1, ENCODING A PROTEIN STRUCTURALLY RELATED TO THE SUBTILISIN-LIKE PROPROTEIN PROCESSING ENZYME FURIN

screening a genomic library of Drosophila melanogaster DNA with a human fur cDNA probe resulted in the isolation of DNA clones that apparently belonged to two different DNA regions of the Drosophila genome. Subsequently, corresponding Drosophila cDNA clones were isolated. Nucleotide sequence analysis indicated that these cDNA clones originated from two different genes, which were called Dfur1 and Dfur2. From overlapping Dfur1 cDNA clones, a composite cDNA could be constructed and analysis of its nucleotide sequence revealed the coding sequence for a protein of 899 amino acid residues. This protein, designated Dfurin 1, exhibited striking sequence homology to human furin and contained the same protein domains except for the cysteine-rich region. Furthermore, unlike human furin, Dfurin 1 possessed an extended amino-terminal region in which a potential transmembrane anchor was present.