CHARACTERIZATION OF DELTA-AMINOLEVULINIC-ACID FORMATION IN SOYBEAN ROOT-NODULES

被引:21
作者
SANGWAN, I
OBRIAN, MR
机构
[1] SUNY BUFFALO,DEPT BIOCHEM,BUFFALO,NY 14214
[2] SUNY BUFFALO,CTR ADV MOLEC BIOL & IMMUNOL,BUFFALO,NY 14214
关键词
D O I
10.1104/pp.98.3.1074
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Formation of the heme precursor delta-aminolevulinic acid (ALA) was studied in soybean root nodules elicited by Bradyrhizobium japonicum. Glutamate-dependent ALA formation activity by soybean (Glycine max) in nodules was maximal at pH 6.5 to 7.0 and at 55 to 60-degrees-C. A low level of the plant activity was detected in uninfected roots and was 50-fold greater in nodules from 17-day-old plants; this apparent stimulation correlated with increases in both plant and bacterial hemes in nodules compared with the respective asymbiotic cells. The glutamate-dependent ALA formation activity was greatest in nodules from 17-day-old plants and decreased by about one-half in those from 38-day-old plants. Unlike the eukaryotic ALA formation activity, B. japonicum ALA synthase activity was not significantly different in nodules than in cultured cells, and the symbiotic activity was independent of nodule age. The lack of symbiotic induction of B. japonicum ALA synthase indicates either that ALA formation is not rate-limiting, or that ALA synthase is not the only source of ALA for bacterial heme synthesis in nodules. Plant cytosol from nodules catalyzed the formation of radiolabeled ALA from U-[C-14]glutamate and 3,4-[H-3]glutamate but not from 1-[C-14]glutamate, and thus, operation of the C5 pathway could not be confirmed.
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页码:1074 / 1079
页数:6
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