CHARACTERIZATION OF CUCUMBER MOSAIC-VIRUS ISOLATES FROM CALIFORNIA

被引:25
作者
DANIELS, J [1 ]
CAMPBELL, RN [1 ]
机构
[1] UNIV CALIF DAVIS, DEPT PLANT PATHOL, DAVIS, CA 95616 USA
关键词
D O I
10.1094/PD-76-1245
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Thirty cucumber mosaic virus (CMV) isolates from California were characterized biologically (host reaction and thermosensitivity), serologically (protein A sandwich-enzyme-linked immunosorbent assay [PAS-ELISA]) and physically (viral capsid protein migration in polyacrylamide gel electrophoresis [PAGE], peptide mapping, and migration of viral double-stranded RNAs [dsRNAs] in PAGE). All methods supported the classification of CMV isolates into two main subgroups, CMV-I and CMV-II. The subgroup CMV-I was divided further into CMV-Ia and CMV-Ib on the basis of host reaction and dsRNA patterns. A low molecular weight RNA 5, presumably a satellite RNA, was detected in two isolates. Peanut stunt virus, tomato aspermy virus, and CMV were distinguished from each other by PAS-ELISA, peptide mapping, and host reaction.
引用
收藏
页码:1245 / 1250
页数:6
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