CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF 3 SEROTYPES OF FOOT-AND-MOUTH-DISEASE VIRUS

被引：21

作者：

CURRY, S

ABUGHAZALEH, R

BLAKEMORE, W

FRY, E

JACKSON, T

KING, A

LEA, S

LOGAN, D

NEWMAN, J

STUART, D

机构：

[1] LAB MOLEC BIOPHYS,REX RICHARDS BLDG,S PARKS RD,OXFORD OX1 3QU,ENGLAND

[2] AFRC,INST ANIM HLTH,PIRBRIGHT LAB,WOKING GU24 0NF,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 228卷 / 04期

关键词：

FOOT-AND-MOUTH DISEASE VIRUS; EMPTY PARTICLES; VIRUS CRYSTALLOGRAPHY; CRYSTALLIZATION; X-RAY DIFFRACTION;

D O I：

10.1016/0022-2836(92)90332-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A1061, A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A1061. In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2S04 or mixtures of polyethylene glycol and NH4Cl. © 1992.

引用

页码：1263 / 1268

页数：6

共 29 条

[1] THE 3-DIMENSIONAL STRUCTURE OF FOOT-AND-MOUTH-DISEASE VIRUS AT 2.9-A RESOLUTION [J].