PURIFICATION AND PROPERTIES OF LECTINS FROM A MUSHROOM, PLEUROTUS-CORNUCOPIAE

被引：29

作者：

YOSHIDA, M

KATO, S

OGURI, S

NAGATA, Y

机构：

[1] Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 03期

关键词：

D O I：

10.1271/bbb.58.498

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hemagglutinating activity in fruit bodies of Pleurotus cornucopiae was separated by DEAE column chromatography into two, adsorbed and unadsorbed, fractions. From the unadsorbed fraction, three active substances were purified and characterized. The main component (PCL-a) consisted of two identical subunits with an apparent molecular mass of 16 kDa and the second (PCL-b) consisted of two heterogeneous subunits of 16 and 15 kDa. The three lectins as well as the two kinds of subunits were immunologically cross-reactive with anti-PCL-a serum. Amino acid compositions of the two subunits were similar, and N-terminal residues of the subunits were blocked. Hemagglutinating activities of the three lectins were not inhibited by any monosaccharide tested but were strongly inhibited by asialo-mucin. From these results, the three lectins in P. cornucopiae were found to be isolectins.

引用

页码：498 / 501

页数：4

共 18 条

[1] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].

DAVIS, BJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&

[2] PRIMARY STRUCTURE OF A FUCOSE-SPECIFIC LECTIN OBTAINED FROM A MUSHROOM, ALEURIA-AURANTIA [J].

FUKUMORI, F ;

TAKEUCHI, N ;

HAGIWARA, T ;

OHBAYASHI, H ;

ENDO, T ;

KOCHIBE, N ;

NAGATA, Y ;

KOBATA, A .

JOURNAL OF BIOCHEMISTRY, 1990, 107 (02) :190-196

[3]

FUKUMORI F, 1990, FEBS LETT, V250, P153

[4]

KANEKO T, 1993, J GEN APPL MICROBIOL, V39, P93

[5] ISOLATION AND CHARACTERIZATION OF A LECTIN FROM GRIFOLA-FRONDOSA FRUITING BODIES [J].

KAWAGISHI, H ;

NOMURA, A ;

MIZUNO, T ;

KIMURA, A ;

CHIBA, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1034 (03) :247-252

[6] PURIFICATION AND PROPERTIES OF A NOVEL FUCOSE-SPECIFIC HEMAGGLUTININ OF ALEURIA-AURANTIA [J].

KOCHIBE, N ;

FURUKAWA, K .

BIOCHEMISTRY, 1980, 19 (13) :2841-2846

[7]

KOCHIBE N, 1989, J BIOL CHEM, V264, P173

[8] SPECIFICITY OF MUSHROOM PLEUROTUS-OSTREATUS AND PLEUROTUS-SPODOLEUCUS EXTRACTS [J].

KOGURE, T .

VOX SANGUINIS, 1975, 29 (03) :221-227

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

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