MOLECULAR-CLONING OF A NOVEL HUMAN CDNA HOMOLOGOUS TO CDC10 IN SACCHAROMYCES-CEREVISIAE

We isolated a novel human cDNA, termed hCDC10, whose predicted product showed a high degree of homology to the CDC10 protein of Saccharomyces cerevisiae. This cDNA contained an open reading frame of 1254 nucleotides encoding 418 amino acids, which included a GTP-binding motif, GX(4)GKS--DX(2)G--KXD. The predicted peptide sequence also, revealed partial amino-acid identity (40-50%) with Diff 6 in Drosophila and with H5 in mouse, Each of these sequence homologues, including Saccharomyces cerevisiae CDC10, contains the GTP-binding motif. Northern blot analyses indicated that the hCDC10 gene is expressed ubiquitously in normal tissues. (C) 1994 Academic Press, Inc.