COLORIMETRIC DETERMINATION OF BIOTIN

被引：22

作者：

PLINTON, C

MAHN, FP

HAWRYLSH.M

VENTUREL.VS

SENKOWSK.BZ

机构：

[1] Analytical Research Laboratory, Quality Control Department, Hoffmann-La Roche Inc., Nutley, New Jersey

[2] Ciba Pharmaceutical Corp, Summit, New Jersey

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 1969年 / 58卷 / 07期

关键词：

Biotin determination; Colorimetric analysis–spectrophotometer; Dicalcium PO[!sub]4[!/sub] preparation–biotin determination;

D O I：

10.1002/jps.2600580719

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A colorimetric method has been developed for the analysis of biotin in premixes with dicalcium phosphate. The method involves the oxidation of the sulfur atom in biotin to the sulfone with potassium iodate in an acid medium and the concurrent reduction of iodate to iodine. The liberated iodine is then extracted into cyclohexane and the absorbance measured at 520 mμ. The relationship between absorbance and the quantity of biotin reacted was found to obey Beer's law over the concentration range studied, 3‐18 mg./g., and the extracted color is stable for several hours. The precision (ts:95% confidence limit) is equal to °5.7%. Copyright © 1969 Wiley‐Liss, Inc., A Wiley Company

引用

页码：875 / &

共 12 条

[1] A MODIFIED MEDIUM FOR LACTOBACILLUS-CASEI FOR THE ASSAY OF VITAMINS-B [J].