QUANTITATION OF LYMPHOCYTE-RESPONSE TO PHA BY FLOW CYTOFLUOROMETRY .3. HETEROGENEITY OF INDUCTION PERIOD

被引：9

作者：

BRAUNSTEIN, JD

SCHWARTZ, G

GOOD, RA

SHARPLESS, TK

MELAMED, MR

机构：

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 01期

关键词：

D O I：

10.1177/27.1.86571

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Early events in phytohaemagglutinin (PHA) stimulation of mouse splenocytes have been quantitated by using flow cytometry and supravital staining with acridine orange (AO). Increasing percentages of single cells with increased metachromatic (red) AO staining were demonstrated in cultures stimulated by PHA for up to 24 hr. These differences in staining could be eliminated by fixation with 1:1 ethanol/acetone before staining. Stimulated cells showed an increase in nonspecific esterase activity as measured by flow cytometry after supravital staining with fluorescein diacetate (FDA). The data reported show a heterogeneity in the per cell response of mouse splenocytes to PHA. The relationship between these data and the mechanism of mitogen stimulation is discussed.

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页码：474 / 477

页数：4

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