ISOLATION OF AN ALIPHATIC AMIDASE FROM PSEUDOMONAS AERUGINOSA

被引：7

作者：

LILLY, MD

CLARKE, PH

HOULDSWORTH, M

CURRIE, JA

DUNNILL, P

机构：

[1] Biochemical Engineering Section, Department of Chemical Engineering, University College, London

[2] Department of Biochemistry, University College London, London

[3] Department of Botany, University College London, London

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1969年 / 11卷 / 03期

关键词：

D O I：

10.1002/bit.260110303

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A pilot‐scale process for the isolation of an aliphatic, amidase from Pseudomonas aeruginosa has been developed. A constitutive, partially irrepressible mutant was employed to give a high initial enzyme concentration. An existing laboratory isolation procedure has been scaled up and modified particularly by substitution of polyethylene glycol for ammonium sulfate precipitation as the first stage in the conversion of the fractionation to continuous operation. Full recovery of activity was achieved with the modification. The recovery of enzyme from a subsequent chromatographic stage was 85% and the maximum overall purification was 28‐fold. Copyright © 1969 John Wiley & Sons, Inc.

引用

页码：283 / +

页数：1

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