CHARACTERIZATION OF PROTEINS AND PEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FLUORESCENCE MONITORING OF THEIR TRYPTIC DIGESTS

被引：58

作者：

RUBINSTEIN, M

CHENKIANG, S

STEIN, S

UDENFRIEND, S

机构：

[1] Roche Institute of Molecular Biology, Nutley

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90193-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins and peptides can be characterized and compared at the subnanomole level by treatment with trypsin followed by high-performance liquid chromatography on reverse-phase partition columns. A fluorescamine monitoring system automatically analyzes a portion of the column effluent while the remainder can be collected for further studies. The method has already been used for characterization of rat β-endorphin and a protein which cross-reacts with antiserum prepared against prolyl hydroxylase. © 1979.

引用

页码：117 / 121

页数：5

共 12 条

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