POLYMERASE CHAIN REACTION-BASED DETECTION OF TRYPANOSOMA-CRUZI DNA IN SERUM

被引：62

作者：

RUSSOMANDO, G ^{[1
]}

FIGUEREDO, A ^{[1
]}

ALMIRON, M ^{[1
]}

SAKAMOTO, M ^{[1
]}

MORITA, K ^{[1
]}

机构：

[1] NAGASAKI UNIV,INST TROP MED,DEPT VIROL,NAGASAKI 852,JAPAN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 11期

关键词：

D O I：

10.1128/JCM.30.11.2864-2868.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNAs prepared from chagasic patients' sera were amplified by the polymerase chain reaction using oligonucleotide primers which anneal specifically to a highly repetitive sequence of Trypanosoma cruzi nuclear DNA. Samples from both acutely and chronically infected patients yielded positive results by this method. No significant difference was observed when either whole blood or serum samples of the patients were used. These results indicate that serum instead of whole-blood samples could be used for polymerase chain reaction-based detection of T. cruzi in field studies without the need of applying any special chemical treatment to the specimens. This would represent a considerable advantage due to the easier handling and transportation of serum as compared with whole-blood samples, especially in tropical climates.

引用

页码：2864 / 2868

页数：5

共 15 条

[1]

ARIAS AR, 1988, T R SOC TROP MED HYG, V82, P248

[2]

AVILA HA, 1991, MOL BIOCHEM PARASIT, V48, P221

[3]

Camargo ME, 1979, TRYPANOSOMA CRUZI DO, P175

[4] IGG ANTIBODY REACTIVITY WITH TRYPANOSOMA-CRUZI AND LEISHMANIA ANTIGENS IN SERA OF PATIENTS WITH CHAGAS-DISEASE AND LEISHMANIASIS [J].

CHILLER, TM ;

SAMUDIO, MA ;

ZOULEK, G .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 43 (06) :650-656

[5]

FREILIJ H, 1983, J CLIN MICROBIOL, V18, P277

[6] MINICHROMOSOMAL REPETITIVE DNA IN TRYPANOSOMA-CRUZI - ITS USE IN A HIGH-SENSITIVITY PARASITE DETECTION ASSAY [J].

GONZALEZ, A ;

PREDIGER, E ;

HUECAS, ME ;

NOGUEIRA, N ;

LIZARDI, PM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (11) :3356-3360

[7]

HASHIGUCHI Y, 1987, STUDIES NEW WORLD LE, P64

[8] DETECTION OF TRYPANOSOMA-CRUZI BY DNA AMPLIFICATION USING THE POLYMERASE CHAIN-REACTION [J].

MOSER, DR ;

KIRCHHOFF, LV ;

DONELSON, JE .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (07) :1477-1482

[9]

PAOLASSO R, 1979, PRENSA MED ARGENT, V66, P594

[10] FETAL IGG SPECIFICITIES AGAINST TRYPANOSOMA-CRUZI ANTIGENS IN INFECTED NEWBORNS [J].

REYES, MB ;

LORCA, M ;

MUNOZ, P ;

FRASCH, ACC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2846-2850

← 1 2 →