RELIABILITY OF DETECTION BY POLYMERASE CHAIN-REACTION OF THE SICKLE CELL-CONTAINING REGION OF THE BETA-GLOBIN GENE IN SINGLE HUMAN BLASTOMERES

被引：32

作者：

PICKERING, SJ

MCCONNELL, JM

JOHNSON, MH

BRAUDE, PR

机构：

[1] Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, Downing Street

[2] Assisted Conception Research Unit, UMDS Department of Obstetrics and Gynaecology, St Thomas' Hospital

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 05期

基金：

英国医学研究理事会;

关键词：

PCR; BETA-GLOBIN; HUMAN BLASTOMERE;

D O I：

10.1093/oxfordjournals.humrep.a137710

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Human preimplantation embryos at various stages of development have been analysed using the polymerase chain reaction to amplify a 680 base pair fragment of the beta-globin gene. Successful amplification was achieved more frequently with DNA from intact embryos containing between one and 11 cells, single cumulus cells, oocytes which had failed to fertilize and polar bodies than from single blastomeres disaggregated from intact embryos and treated in an identical manner. The distribution of nuclei demonstrated using the nuclear chromophore diamino-phenyl-indole showed considerable inter-blastomere variation; however, no clear correlation between staining pattern and successful amplification was observed. The reason for the unreliable amplification of DNA from single blastomeres is unclear but this finding has important implications for preimplantation diagnosis of genetic disease.

引用

页码：630 / 636

页数：7

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