INHIBITION OF HUMAN CYTOMEGALOVIRUS MAJOR IMMEDIATE-EARLY GENE-EXPRESSION BY ANTISENSE RNA EXPRESSION VECTORS

被引：13

作者：

BRYANT, LA ^{[1
]}

SINCLAIR, JH ^{[1
]}

机构：

[1] UNIV CAMBRIDGE, ADDENBROOKES HOSP, DEPT MED, CAMBRIDGE CB2 2QQ, ENGLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷

基金：

英国医学研究理事会;

关键词：

D O I：

10.1099/0022-1317-74-9-1965

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have used antisense oligonucleotides and expression vectors to inhibit human cytomegalovirus (HCMV) major immediate early (IE) gene expression. We find that oligonucleotides complementary to the HCMV 72K IE protein (IE1) coding region do inhibit HCMV infection, but this is non-specific. However, the use of certain antisense expression vectors, which express short oligonucleotides complementary to IE1. specifically inhibits IE1 expression at the protein level after introduction of IE expression vectors into cells or after HCMV infection.

引用

页码：1965 / 1967

页数：3

共 19 条

[1] THE STRUCTURE OF THE MAJOR IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS STRAIN AD169 [J].

AKRIGG, A ;

WILKINSON, GWG ;

ORAM, JD .

VIRUS RESEARCH, 1985, 2 (02) :107-121

[2]

ALFORD CA, 1990, VIROLOGY, P1981

[3] A VERY STRONG ENHANCER IS LOCATED UPSTREAM OF AN IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS [J].

BOSHART, M ;

WEBER, F ;

JAHN, G ;

DORSCHHASLER, K ;

FLECKENSTEIN, B ;

SCHAFFNER, W .

CELL, 1985, 41 (02) :521-530

[4] HUMAN CYTOMEGALOVIRUS-IE1 TRANSACTIVATES THE ALPHA-PROMOTER-ENHANCER VIA AN 18-BASE-PAIR REPEAT ELEMENT [J].

CHERRINGTON, JM ;

MOCARSKI, ES .

JOURNAL OF VIROLOGY, 1989, 63 (03) :1435-1440

[5] THE 72K IE1 AND 80K IE2 PROTEINS OF HUMAN CYTOMEGALOVIRUS INDEPENDENTLY TRANSACTIVATE THE C-FOS, C-MYC AND HSP70 PROMOTERS VIA BASAL PROMOTER ELEMENTS [J].

HAGEMEIER, C ;

WALKER, SM ;

SISSONS, PJG ;

SINCLAIR, JH .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2385-2393

[6] INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 MULTIPLICATION BY ANTISENSE AND SENSE RNA EXPRESSION [J].

JOSHI, S ;

VANBRUNSCHOT, A ;

ASAD, S ;

VANDERELST, I ;

READ, SE ;

BERNSTEIN, A .

JOURNAL OF VIROLOGY, 1991, 65 (10) :5524-5530

[7] THE 21BP REPEAT ELEMENT OF THE HUMAN CYTOMEGALOVIRUS MAJOR IMMEDIATE EARLY ENHANCER IS A NEGATIVE REGULATOR OF GENE-EXPRESSION IN UNDIFFERENTIATED CELLS [J].

KOTHARI, S ;

BAILLIE, J ;

SISSONS, JGP ;

SINCLAIR, JH .

NUCLEIC ACIDS RESEARCH, 1991, 19 (08) :1767-1771

[8] TRANSACTIVATION OF A HUMAN CYTOMEGALOVIRUS EARLY PROMOTER BY GENE-PRODUCTS FROM THE IMMEDIATE-EARLY GENE IE2 AND AUGMENTATION BY IE1 - MUTATIONAL ANALYSIS OF THE VIRAL-PROTEINS [J].

MALONE, CL ;

VESOLE, DH ;

STINSKI, MF .

JOURNAL OF VIROLOGY, 1990, 64 (04) :1498-1506

[9]

MATSUKURA M, 1987, P NATIONAL ACADEMY S, V84, P1210

[10] TRANS-ACTIVATION AND AUTO-REGULATION OF GENE-EXPRESSION BY THE IMMEDIATE-EARLY REGION-2 GENE-PRODUCTS OF HUMAN CYTOMEGALO-VIRUS [J].

PIZZORNO, MC ;

OHARE, P ;

SHA, L ;

LAFEMINA, RL ;

HAYWARD, GS .

JOURNAL OF VIROLOGY, 1988, 62 (04) :1167-1179

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