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CHARACTERIZATION OF THINOPYRUM-BESSARABICUM CHROMOSOME SEGMENTS IN WHEAT USING RANDOM AMPLIFIED POLYMORPHIC DNAS (RAPDS) AND GENOMIC IN-SITU HYBRIDIZATION

被引：63
作者：
KING, IP
PURDIE, KA
REZANOOR, HN
KOEBNER, RMD
MILLER, TE
READER, SM
NICHOLSON, P
机构：
[1] Cambridge Laboratory, IPSR, JI Centre, Colney, Norwich, NR4 7UJ, Norfolk
来源：
THEORETICAL AND APPLIED GENETICS | 1993年 / 86卷 / 08期
关键词：
RANDOM AMPLIFIED POLYMORPHIC DNA; GENOMIC IN-SITU HYBRIDIZATION; WHEAT; THINOPYRUM-BESSARABICUM; ISOZYMES;
D O I：
10.1007/BF00211038
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Ten random amplified polymorphic DNA (RAPD) markers specific to chromosome 5E(b) of Thinopyrum bessarabicum were detected. Genomic in situ hybridization and standard cytological observations revealed that six of the markers are located on the 5E(b) short arm and four are located on the 5E(b) long arm. These RAPD markers have been used to confirm the identity of putative 5E(b) (5A) and 5E(b) (5D) substitution individuals. The potential of RAPDs for the detection of wheat/alien recombinants is discussed.
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页码：895 / 900
页数：6
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