CLONING AND CHARACTERIZATION OF 2 CDNA CLONES ENCODING SEED-SPECIFIC ANTIMICROBIAL PEPTIDES FROM MIRABILIS-JALAPA L

被引：22

作者：

DEBOLLE, MFC

EGGERMONT, K

DUNCAN, RE

OSBORN, RW

TERRAS, FRG

BROEKAERT, WF

机构：

[1] CATHOLIC UNIV LEUVEN,F A JANSSENS LAB GENET,B-3001 HEVERLEE,BELGIUM

[2] ZENECA AGROCHEM,JEALOTTS HILL RES STN,BRACKNELL RG12 6EY,BERKS,ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1995年 / 28卷 / 04期

关键词：

ANTIFUNGAL PEPTIDE; ANTIMICROBIAL PEPTIDE; CDNA CLONE; GENOMIC CLONE; MIRABILIS JALAPA; SEED-SPECIFIC;

D O I：

10.1007/BF00021195

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and characterized two cDNA clones (designated MJ1 and MJ2) encoding the two Mirabilis jalapa antimicrobial peptides (M(j)-AMP1 and M(j)-AMP2, respectively), which were previously purified from seeds of this plant species (Cammue et al. (1992), J Biol Chem 267: 2228-2233). In both cases, the deduced amino acid sequences reveal the presence of a putative signal sequence preceding the mature peptide, indicating that the M(j)-AMPs are expressed as preproteins. The M(j)-AMP1- and M(j)-AMP2-encoding genes are interrupted in their coding sequences by a single intron (380 bp and 900 bp for M(j)-AMP1 and M(j)-AMP2 genes, respecticely). Southern blot analysis indicates that the Mj-AMP-encoding genes belong to a gene family of low complexity. Northern blot analysis suggests seed-specific expression of M(j)-AMPs since transcripts of the expected size could only be detected in near-mature and in mature seeds of M. jalapa.

引用

页码：713 / 721

页数：9

共 34 条

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