HUMAN OOCYTES - MATURATION IN CHEMICALLY DEFINED MEDIA

被引：83

作者：

KENNEDY, JF

DONAHUE, RP

机构：

[1] Division of Population Dynamics, Department of Gynecology and Obstetrics, Johns Hopkins University, Baltimore

来源：

SCIENCE | 1969年 / 164卷 / 3885期

关键词：

D O I：

10.1126/science.164.3885.1292

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human oocytes from ovarian follicles resume meiosis in F10, a defined medium, in numbers comparable to that obtained in medium containing serum. Several simple Krebs-Ringer media also support maturation, which suggests a similarity of nutritional requirements between human and mouse oocytes. Fewer oocytes reach metaphase II when the investing follicular cells are removed prior to culture.

引用

页码：1292 / &

共 9 条

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