INFLUENCE OF SECRETIN, GLUCAGON AND OTHER PEPTIDES, OF AMINO-ACIDS, PROSTAGLANDIN ENDOPEROXIDE ANALOGS AND DIAZEPAM ON THE LEVEL OF ADENOSINE 3',5'-CYCLIC-MONOPHOSPHATE IN NEUROBLASTOMA-GLIOMA HYBRID-CELLS

Abstract— Neuroblastoma glioma hybrid cells display many properties of neurons. A series of compounds, among them a number of amino acids, peptides and peptide hormones were tested for their ability to influence the level of adenosine 3′,5′‐cyclic monophosphate (cyclic AMP) in the hybrid cells. Two prostaglandin endoperoxide analogues exhibit a weak stimulatory action, if applied in at least micromolar concentrations. At nanomolar concentrations, only the gastrointestinal hormones secretin and glucagon stimulate the formation of cyclic AMP, as detected in the presence of a phosphodiesterase inhibitor. The effect of secretin but not that of glucagon is antagonized by secretin‐(5–27), suggesting that secretin and glucagon act on the cells via different receptors. These results appear to be noteworthy since (a) an effect of secretin or glucagon on a cell with neuronal characteristics has not yet been described, and (b) many peptide hormones have been detected both in the gastrointestinal tract and the nervous system. Copyright © 1979, Wiley Blackwell. All rights reserved