STUDIES ON NUCLEAR PROTEINS - BINDING OF EXTRA ACIDIC PROTEINS TO DEOXYRIBONUCLEOPROTEIN DURING PREPARATION OF NUCLEAR PROTEINS

Acidic proteins can be removed from calf thymus deoxyribonucleoprotein, previously washed with 0.14 M NaCl, using 0.35 M NaCl. After the removal of these acidic proteins, the yield of acidic nuclear proteins as extracted by other methods dropped to less than one third. Calf thymus deoxyribonucleoprotein and reconstituted deoxyribonucleohistone adsorb acidic proteins from the cytoplasm and nuclear sap in 0.14 M NaCl. These proteins which can be released in 0.35 M NaCl are similar in amino acid analysis to the acidic nuclear proteins prepared by other methods. Copyright © 1969, Wiley Blackwell. All rights reserved