FIELD INVERSION GEL-ELECTROPHORESIS WITH DIFFERENT PULSE TIME RAMPS

被引：16

作者：

HELLER, C ^{[1
]}

POHL, FM ^{[1
]}

机构：

[1] UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 21期

关键词：

D O I：

10.1093/nar/18.21.6299

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of different pulse time ramps on the separation of yeast chromosomes with field inversion gel electrophoresis (FIGE) was investigated by the means of two dimensional gel electrophoresis. The problem of band inversion, which makes it difficult to distinguish DNA molecules of different size, has been solved by using double randomized pulse times. A major disadvantage of the field inversion technique is thereby overcome, making this system comparable to other pulsed field techniques. © 1990 Oxford University Press.

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页码：6299 / 6304

页数：6

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