LIMITED DIVISION OF LOW-DENSITY ADULT-RAT TYPE-II PNEUMOCYTES IN SERUM-FREE CULTURE

被引:31
作者
TANSWELL, AK
BYRNE, PJ
HAN, RNN
EDELSON, JD
HAN, VKM
机构
[1] UNIV TORONTO,ST MICHAELS HOSP,DEPT MED,TORONTO M5B 1W8,ONTARIO,CANADA
[2] UNIV WESTERN ONTARIO,LAWSON RES INST,DEPT ANAT,LONDON N6A 4V2,ONTARIO,CANADA
[3] UNIV WESTERN ONTARIO,LAWSON RES INST,DEPT PAEDIAT,LONDON N6A 4V2,ONTARIO,CANADA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 260卷 / 06期
关键词
INSULIN-LIKE GROWTH FACTORS; SERUM-FREE MEDIUM;
D O I
10.1152/ajplung.1991.260.6.L395
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
A previously reported serum-free medium for the culture of adult rat type II pneumocytes has been further defined by the addition of 10 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid buffer and 700 U/ml catalase. Both additions significantly enhanced [H-3]thymidine incorporation into pneumocyte DNA between days 5 and 6 of culture. In the presence of 100-mu-g/ml bovine pituitary extract, both insulin-like growth factor (IGF) I (20 ng/ml) and II (25 ng/ml) further increased [H-3]thymidine incorporation into pneumocyte DNA over that observed with the basal medium. When type II pneumocytes were plated at 2.75 x 10(5) cells/cm2 there was a plating efficiency of 50 +/- 5%, and the increased DNA synthesis in response to IGFs was not associated with an increase in cell number. When the plating number was reduced to 1.6 x 10(5) cells/cm2 the plating efficiency was only 23 +/- 2%. At this low cell density a doubling of cell number was observed in response to IGF-I between days 4 and 9 of culture.
引用
收藏
页码:L395 / L402
页数:8
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